1. Recombinant Proteins
  2. CAR-T Related Proteins Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 9 (CA IX)
  5. Carbonic Anhydrase 9 Protein, Canine (HEK293, His)

Carbonic Anhydrase 9 Protein, Canine (HEK293, His)

Cat. No.: HY-P75454
COA Handling Instructions

The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Canine (HEK293, His) is the recombinant canine-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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5 μg $90 In-stock
10 μg $145 In-stock
20 μg $230 In-stock
50 μg $435 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Canine (HEK293, His) is the recombinant canine-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag.

Background

Carbonic Anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion between carbon dioxide and water. It facilitates the formation of bicarbonate and hydrogen ions, the dissociated ions of carbonic acid. This enzymatic activity is essential for maintaining proper pH balance and regulating various physiological processes, including acid-base homeostasis, respiration, and ion transport. CA9 protein acts as a key player in the dynamic equilibrium of carbon dioxide and water, contributing to the efficient transport and regulation of these molecules in the body.

Biological Activity

Measured by its esterase activity. The specific activity is 11.69 pmol/min/μg that incubate at room temperature in kinetic mode for 5 minutes.

Species

Canine

Source

HEK293

Tag

C-6*His

Accession

NP_001138646.1 (Q38-L410)

Gene ID
Molecular Construction
N-term
CA9 (Q38-L410)
Accession # NP_001138646.1
His
C-term
Synonyms
Carbonic anhydrase 9; CA-IX; P54/58N; pMW1; CA9; G250; MN
AA Sequence

QSLSRIQGTPGLGGDSSGEDDQLDEENLPSEEDPHGEEDPPGEEDPPGEEDPPGEEDPSGMKTEPGKEDSLKLEDLPTVEAPRDTPGSQNNAHRHNKGDDHSHWRYGGDPPWPQVSPACAGRFQSPVDIRPQLAALSPALPPLELLGFELPPLPELRLRNNGHTVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVDGHRFPAEIHVVHISTAFAKVEEALGRPGGLAVLAAFLQEGPEENSAYEQLLSHLEEITEEDSETWVPGLDVSALLPSDLSRYFRYEGSLTTPPCAQGVIWTVFNQTVRLSAKQLHTLSGSLWGPDDSRLQLNFRATQPLNGRIIEASFPAQVESSPRSFEPVHLNSCL

Molecular Weight

Approximately 42-55 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Carbonic Anhydrase 9 Protein, Canine (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Carbonic Anhydrase 9 Protein, Canine (HEK293, His)
Cat. No.:
HY-P75454
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