Carbonic Anhydrase 7 Protein, Human (His)

Carbonic Anhydrase VII/CA7 Protein, Human (His) expresses in E. coli with a His tag at the N-terminus. Human carbonic anhydrase VII (hCA VII) is a cytosolic isoform belonging to the α-CA family that shows high carbon dioxide hydration activity^[1].

hCA VII has also been reported to be involved in the initial postnatal phases of brain development, as well as in the generation of neuronal excitation and febrile seizures^[1].

Measured by its esterase activity. The specific activity is 102.87 pmol/min/µg that incubate at room temperature in kinetic mode for 5 minutes.

Human

E. coli

C-6*His

P43166 (M1-A264)

766  [NCBI]

MTGHHGWGYGQDDGPSHWHKLYPIAQGDRQSPINIISSQAVYSPSLQPLELSYEACMSLSITNNGHSVQVDFNDSDDRTVVTGGPLEGPYRLKQFHFHWGKKHDVGSEHTVDGKSFPSELHLVHWNAKKYSTFGEAASAPDGLAVVGVFLETGDEHPSMNRLTDALYMVRFKGTKAQFSCFNPKCLLPASRHYWTYPGSLTTPPLSESVTWIVLREPICISERQMGKFRSLLFTSEDDERIHMVNNFRPPQPLKGRVVKASFRAHHHHHH

Approximately 31.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Simona M.Monti, et al, et al.Chapter 9 - Carbonic Anhydrase VII. Carbonic Anhydrases as Biocatalysts. From Theory to Medical and Industrial Applications, 2015, Pages 151-168