Carboxylesterase 5 Protein, Mouse (HEK293, His)

Carboxylesterase 5 Protein, Mouse (HEK293, His) is a mouse Carboxylesterase 5 protein with a His-flag. Carboxylesterase 5 is encoded byCarboxylesterase 5 (CES5) and can catalyses hydrolytic and transesterification reactions^[1][2].

Carbonic Anhydrase 11 belongs to the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide and thus participate in a variety of biological and physical processes^[1].
Carboxylesterase 5 (CES5) is one of at least five mammalian CES gene families encoding enzymes of broad substrate specificity and catalysing hydrolytic and transesterification reactions^[2].

Measured by its ability to hydrolyze 1mM 4-Nitrophenyl acetate that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 10573.649- 37513.038 pmol/min/μg.

Mouse

HEK293

C-6*His

Q8BK48 (Q27-R555)

234673  [NCBI]

QDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNLPVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQNIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGCAAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWILPTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPALQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQERHHHHHHH

58-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 150 mM NaCl, pH8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Roger S Holmes, et al. Mammalian carboxylesterase 5: comparative biochemistry and genomics. 2008 Sep;3(3):195-204.  [Content Brief]