1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. CBR3 Protein, Human (His)

CBR3 Protein, Human (His)

Cat. No.: HY-P75605
COA Handling Instructions

The CBR3 protein uses NADPH to convert carbonyl compounds into alcohols. CBR3 Protein, Human (His) is the recombinant human-derived CBR3 protein, expressed by E. coli , with N-His labeled tag. The total length of CBR3 Protein, Human (His) is 277 a.a., with molecular weight of ~37 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The CBR3 protein uses NADPH to convert carbonyl compounds into alcohols. CBR3 Protein, Human (His) is the recombinant human-derived CBR3 protein, expressed by E. coli , with N-His labeled tag. The total length of CBR3 Protein, Human (His) is 277 a.a., with molecular weight of ~37 kDa.

Background

CBR3, a multifaceted enzyme, catalyzes the NADPH-dependent reduction of various carbonyl compounds, transforming them into their respective alcohols. Although it exhibits low NADPH-dependent oxidoreductase activity, CBR3 is particularly effective on orthoquinones, including 1,2-naphthoquinone, showcasing its potential role in protecting against the cytotoxic effects of exogenous quinones. The enzyme is not limited to quinones, as it also acts on non-quinone compounds like isatin and the anticancer drug oracin. CBR3's preference for ortho-quinones over paraquinones highlights its substrate specificity. Notably, no endogenous substrate, aside from isatin, has been identified for CBR3, underscoring its versatility and potential contributions to cellular detoxification processes.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-His

Accession

O75828 (M1-W277)

Gene ID

874  [NCBI]

Molecular Construction
N-term
His
CBR3 (M1-W277)
Accession # O75828
C-term
Synonyms
Carbonyl reductase [NADPH] 3; NADPH-dependent carbonyl reductase 3; CBR3
AA Sequence

MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

Molecular Weight

Approximately 37 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CBR3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CBR3 Protein, Human (His)
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