CBR3 Protein, Human (His)

The CBR3 protein uses NADPH to convert carbonyl compounds into alcohols. CBR3 Protein, Human (His) is the recombinant human-derived CBR3 protein, expressed by E. coli , with N-His labeled tag. The total length of CBR3 Protein, Human (His) is 277 a.a., with molecular weight of ~37 kDa.

CBR3, a multifaceted enzyme, catalyzes the NADPH-dependent reduction of various carbonyl compounds, transforming them into their respective alcohols. Although it exhibits low NADPH-dependent oxidoreductase activity, CBR3 is particularly effective on orthoquinones, including 1,2-naphthoquinone, showcasing its potential role in protecting against the cytotoxic effects of exogenous quinones. The enzyme is not limited to quinones, as it also acts on non-quinone compounds like isatin and the anticancer drug oracin. CBR3's preference for ortho-quinones over paraquinones highlights its substrate specificity. Notably, no endogenous substrate, aside from isatin, has been identified for CBR3, underscoring its versatility and potential contributions to cellular detoxification processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

O75828 (M1-W277)

874  [NCBI]

MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.