1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-1 gamma/CCL9/CCL10
  6. CCL9/10/MIP-1 gamma Protein, Mouse

CCL9/10/MIP-1 gamma Protein, Mouse

Cat. No.: HY-P7772
COA Handling Instructions

CCL9 Protein, Mouse, is often produced in macrophages, osteoclasts, and functions as a cell survival factor.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
2 μg $45 In-stock
10 μg $115 In-stock
50 μg $345 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

CCL9 Protein, Mouse, is often produced in macrophages, osteoclasts, and functions as a cell survival factor.

Background

CCL9, a chemokine also known as macrophage inflammatory protein-1 gamma (MIP-1γ), is often produced in macrophages, osteoclasts, and functions as a cell survival factor. Among CC chemokine family members, the mouse CCL9 shares 45, 24, and 20% aa sequence identity with mouse CCL6 (C10), CCL3 (MIP-1α), and CCL4 (MIP-1β), respectively. CCL9/CCR1 signaling is important for the recruitment of myeloid progenitors to intestinal tumors, leading to enhanced invasion. CCL9 is highly induced in immature myeloid cells in the premetastatic lung of tumor-bearing mice or in co-culture with tumor cells, and it plays a critical role in tumor cell survival at the metastatic site[1][2].

Biological Activity

1.The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/mL.
2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is ≤0.34 μg/mL, corresponding to a specific activity is ≥2.94×103 U/mg.

  • Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.34 μg/mL, corresponding to a specific activity is 2.94×103 U/mg.
Species

Mouse

Source

E. coli

Tag

Tag Free

Accession

P51670 (Q22-Q122)

Gene ID
Molecular Construction
N-term
CCL9 (Q22-Q122)
Accession # P51670
C-term
Synonyms
rMuCCL9; C-C motif chemokine 9; Macrophage inflammatory protein 1-gamma; Small-inducible cytokine A9; Scya9; CCL9.
AA Sequence

QITHATETKEVQSSLKAQQGLEIEMFHMGFQDSSDCCLSYNSRIQCSRFIGYFPTSGGCTRPGIIFISKRGFQVCANPSDRRVQRCIERLEQNSQPRTYKQ

Molecular Weight

Approximately 12-15 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 300 mM NaCl, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

CCL9/10/MIP-1 gamma Protein, Mouse Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CCL9/10/MIP-1 gamma Protein, Mouse
Cat. No.:
HY-P7772
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