CCN2/CTGF Protein, Rat (HEK293)

The CCN2/CTGF protein is a major connective tissue mitochondrial attractant secreted by vascular endothelial cells and critical for chondrocyte proliferation and differentiation. Additionally, it mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. CCN2/CTGF Protein, Rat (HEK293) is the recombinant rat-derived CCN2/CTGF protein, expressed by HEK293 , with tag free.

CCN2/CTGF protein, a major connective tissue mitoattractant, is secreted by vascular endothelial cells. It plays a crucial role in promoting the proliferation and differentiation of chondrocytes. Additionally, CCN2/CTGF mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. Furthermore, it enhances fibroblast growth factor-induced DNA synthesis. CCN2/CTGF exists as a monomer and interacts with TSKU.

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED₅₀ for this effect is 1.921 μg/mL

• 
  Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED₅₀ for this effect is 1.921 μg/mL, corresponding to a specific activity is 521 units/mg.

Rat

HEK293

Tag Free

Q9R1E9 (Q25-A347)

64032  [NCBI]

QDCSAQCQCAAEAAPRCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTVVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

Approximately 35-43 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.