CD161 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

CD161 Protein plays a crucial role in inhibiting NK cell cytotoxicity by activating specific acid sphingomyelinase/SMPD1, elevating ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T-cell proliferation. As a lectin, CD161 binds to Gal-alpha(1,3)Gal and N-acetyllactosamine epitopes, acting as a ligand for CLEC2D/LLT1. Existing as a homodimer, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted immune regulatory functions. CD161 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus-derived CD161 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD161 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is 161 a.a., with molecular weight of 37-42 kDa.

CD161 assumes a crucial role in inhibiting natural killer (NK) cell cytotoxicity. Upon activation, CD161 stimulates specific acid sphingomyelinase/SMPD1, resulting in a significant increase in intracellular ceramide levels. The activation process also leads to the stimulation of AKT1/PKB and RPS6KA1/RSK1 kinases, along with a marked enhancement of T-cell proliferation induced by anti-CD3. Functioning as a lectin, CD161 binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and the N-acetyllactosamine epitope. Furthermore, it acts as a ligand for CLEC2D/LLT1, inhibiting NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells. Existing as a homodimer with disulfide linkage, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted regulatory functions in immune responses^[1][2][3].

Cynomolgus

HEK293

C-Avi;C-His

XP_005570142 (Q67-L227)

102139342  [NCBI]

37-42 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.