CD161 Protein, Human (Biotinylated, HEK293, His-Avi)

CD161 protein plays a crucial role in inhibiting NK cell toxicity by activating specific acid sphingomyelinase/SMPD1 and increasing ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T cell proliferation. CD161 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD161 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD161 Protein, Human (Biotinylated, HEK293, His-Avi) is 159 a.a., with molecular weight of 35-43 kDa.

CD161 Protein assumes a crucial role in inhibiting natural killer (NK) cell cytotoxicity. Upon activation, CD161 stimulates specific acid sphingomyelinase/SMPD1, resulting in a significant increase in intracellular ceramide levels. The activation process also leads to the stimulation of AKT1/PKB and RPS6KA1/RSK1 kinases, along with a marked enhancement of T-cell proliferation induced by anti-CD3. Functioning as a lectin, CD161 binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and the N-acetyllactosamine epitope. Furthermore, it acts as a ligand for CLEC2D/LLT1, inhibiting NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells. Existing as a homodimer with disulfide linkage, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted regulatory functions in immune responses.

1.Immobilized Biotinylated Human CD161 His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD161 Antibody hFc with the EC₅₀ < 9.3 ng/mL determined by ELISA.
2.Immobilized Ant-CD161 Anlibody,hFc Tag at 5 μg/mL (100 μL/Well)on the plate. Dose response curve for Biotinylated Human CD161,His Tag with the EC₅₀ ≤ 28.9 ng/mL detormined by ELISA.

Human

HEK293

C-Avi;C-His

Q12918 (Q67-S225)

3820  [NCBI]

35-43 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.