CD163 Protein, Mouse (HEK293, His)

CD163 protein is pivotal in macrophages for clearing and endocytosing hemoglobin/haptoglobin complexes, safeguarding tissues from oxidative damage. It contributes to iron recycling by facilitating complex endocytosis and heme breakdown, with calcium and pH influencing hemoglobin binding. This initiates intracellular signals involving tyrosine kinase, calcium mobilization, and cytokine secretion. The soluble form, sCD163, released through shedding, may have an anti-inflammatory role. CD163 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD163 protein, expressed by HEK293, with C-His labeled tag. The total length of CD163 Protein, Mouse (HEK293, His) is 1007 a.a., with molecular weight of 120-150 kDa.

CD163 protein is intricately involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes within macrophages, serving a crucial function in protecting tissues from potential oxidative damage caused by free hemoglobin. Additionally, CD163 may contribute to the uptake and recycling of iron by facilitating the endocytosis of hemoglobin/haptoglobin complexes and subsequent breakdown of heme. The protein exhibits a calcium-dependent and pH-dependent binding affinity for hemoglobin/haptoglobin complexes, initiating a cascade of intracellular signals involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and the secretion of interleukin-6 (IL6) and colony-stimulating factor 1 (CSF1). Furthermore, the soluble form of CD163 (sCD163), released through shedding, may exert an anti-inflammatory role.

Mouse

HEK293

C-His

Q2VLH6-1 (V39-T1045)

/

120-150 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.