1. Recombinant Proteins
  2. Immune Checkpoint Proteins Receptor Proteins
  3. Stimulatory Immune Checkpoint Molecules
  4. CD200R
  5. CD200R1
  6. CD200R1 Protein, Human (HEK293, Fc)

The CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein to limit inflammation by inhibiting TNF-α, interferon, and iNOS. Interestingly, CD200R1 displays comparable affinity and kinetics to the human herpesvirus 8 K14 viral CD200 homologue, reflecting its host CD200 interaction. CD200R1 Protein, Human (HEK293, Fc) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-hFc labeled tag.

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Size Price Stock Quantity
20 μg USD 125 In-stock
50 μg USD 275 In-stock
100 μg USD 467 Get quote
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein to limit inflammation by inhibiting TNF-α, interferon, and iNOS. Interestingly, CD200R1 displays comparable affinity and kinetics to the human herpesvirus 8 K14 viral CD200 homologue, reflecting its host CD200 interaction. CD200R1 Protein, Human (HEK293, Fc) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CD200R1, an inhibitory receptor, acts as a pivotal modulator by binding to the CD200/OX2 cell surface glycoprotein. Its regulatory role extends to limiting inflammation through the suppression of pro-inflammatory molecules, including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS), in response to specific stimuli. Intriguingly, CD200R1 exhibits comparable affinity and kinetics when binding to the Human herpesvirus 8 K14 viral CD200 homolog, mirroring its interaction with the host CD200. The interaction between CD200 and CD200R1 is facilitated through their respective N-terminal Ig-like domains, emphasizing the significance of this molecular interplay. Moreover, CD200R1 engages with the Human herpesvirus 8 vOX2 protein, further expanding its versatile interactions in cellular processes.

Biological Activity

Measured by its binding ability in a functional ELISA. When Human CD200 is immobilized at 0.5 µg/mL (100 µL/well), Human CD200 R1 binds with an EC50 of 26.6 ng/mL.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q8TD46-4 (A27-L266)

Gene ID
Molecular Construction
N-term
CD200R1 (A27-L266)
Accession # Q8TD46-4
hFc
C-term
Synonyms
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor
Molecular Weight

53.7 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD200R1 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
CD200R1 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P77599
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MCE Japan Authorized Agent: