CD316/IGSF8 Protein, Human (HEK293, His)

The CD316/IGSF8 protein plays a key role in multiple functions related to CD81 and CD9, affecting processes such as oocyte fertilization, hepatitis C virus function, and the regulation of keratinocyte proliferation and differentiation. CD316/IGSF8 Protein, Human (HEK293, His) is the recombinant human-derived CD316/IGSF8 protein, expressed by HEK293 , with C-6*His labeled tag.

The CD316/IGSF8 protein appears to play a key role in diverse functions associated with CD81 and CD9, such as oocyte fertilization and the function of the hepatitis C virus. Additionally, it may regulate the proliferation and differentiation of keratinocytes, acting as a negative regulator of cell motility. CD316/IGSF8 has been found to suppress T-cell mobility in coordination with CD81, associate with CD82 to suppress prostate cancer cell migration, and regulate epidermoid cell reaggregation and motility on laminin-5, with CD9 and CD81 acting as key linkers in these processes. Furthermore, CD316/IGSF8 may be involved in integrin-dependent morphology and motility functions and participate in the regulation of neurite outgrowth, contributing to the maintenance of the neural network in the adult brain. Its direct interactions with CD82, CD81/tetraspanin-28, and CD9/tetraspanin-29, as well as its interactions with integrin alpha-3/beta-1 and integrin alpha-4/beta-1, underscore the complex molecular associations that contribute to its multifaceted roles in various cellular processes.

Human

HEK293

C-6*His

Q969P0-1 (R28-T579)

93185  [NCBI]

REVLVPEGPLYRVAGTAVSISCNVTGYEGPAQQNFEWFLYRPEAPDTALGIVSTKDTQFSYAVFKSRVVAGEVQVQRLQGDAVVLKIARLQAQDAGIYECHTPSTDTRYLGSYSGKVELRVLPDVLQVSAAPPGPRGRQAPTSPPRMTVHEGQELALGCLARTSTQKHTHLAVSFGRSVPEAPVGRSTLQEVVGIRSDLAVEAGAPYAERLAAGELRLGKEGTDRYRMVVGGAQAGDAGTYHCTAAEWIQDPDGSWAQIAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPAGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGVGSLGPGYEGRHIAMEKVASRTYRLRLEAARPGDAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEDGELSSVPAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHSLGPEDEGVYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYMHALDT

70-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.