CD34 Protein, Rat (HEK293, Fc)

CD34 Protein is a transmembrane phosphoglycoprotein that exists on the surface of human and various animal cells. CD34 Protein is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 Protein plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response. CD34 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD34 protein, expressed by HEK293 , with C-hFc labeled tag.

CD34 is a transmembrane phosphoglycoprotein present on the surface of human and various animal cells, and it was first described in hematopoietic stem cells as an intercellular adhesion factor. CD34 is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 can mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. CD34 may also play a more selective role in the chemokine-dependent migration of eosinophils and dendritic cell precursors. CD34 plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response^[1][2][3][4].

Rat

HEK293

C-hFc

B1PLB1 (E37-T291)

305081  [NCBI]

ENSTQGVFPPVPTNESTEESITSSIPGSTSHYYLIGQDSNKTTPAISETTVNFTATPGTPSGSGTPHTFSQPQTYPTVTLPTTSDSTSTPEVEHSTWNTSLPSVNVSDYSPNNVSSEMISTTEPCAHTSSSVPSIIKGDIKCSGIRGVPFTQGICLELSEASSCEEFKKERGEELMQILCKKEETEADAGTSVCSLNLAQSEVRPECLLMVLANSTELSSKLQLMEKHQSDLRKLGIQSFNKQDLGSHQSYSRKT

Approximately 95-130 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.