CD3E-CD3G Heterodimer Protein, Rhesus Macaque (HEK293, Fc)

CD3E protein has predicted functions such as SH3 domain binding and protein heterodimerization, and plays a crucial role in nervous system development and cell adhesion. It is involved in T cell activation, cytokine production and signal transduction. CD3E-CD3G Heterodimer Protein, Rhesus Macaque (HEK293, Fc) is a recombinant protein dimer complex containing Rhesus Macaque-derived CD3E-CD3G Heterodimer protein, expressed by HEK293 , with C-hFc labeled tag. CD3E-CD3G Heterodimer Protein, Rhesus Macaque (HEK293, Fc), has molecular weight of ~83 (40 + 43) KDa.

The CD3E protein, predicted to possess various functions such as SH3 domain binding activity, identical protein binding activity, and protein heterodimerization activity, plays a crucial role in nervous system development and the positive regulation of cell adhesion. It is actively involved upstream of multiple processes, including positive regulation of T cell activation, positive regulation of cytokine production, and regulation of signal transduction. The protein is located in diverse cellular components, including dendritic spines, the external side of the plasma membrane, and immunological synapses. As part of the alpha-beta T cell receptor complex, its expression is observed in the colon and hemolymphoid system. The human ortholog of this gene, CD3E, has implications in immunodeficiency 18, highlighting its significance in immune system functionality. Biased expression is evident in tissues such as the thymus and spleen, underscoring its role in immune processes.

Immobilized CD3E&CD3G Heterodimer Protein, at 2μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody. The ED₅₀ for this effect is 1.644 ng/mL when detected by Biotinylated Human BCMA.

• 
  Immobilized CD3E&CD3G Heterodimer Protein, at 2μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody .The ED₅₀ for this effect is 1.644 ng/mL when detected by Biotinylated Human BCMA.

Rhesus Macaque

HEK293

C-hFc

XP_014971302 (Q22-D117)&NP_001253854 (Q23-T113)

699467  [NCBI]&705270  [NCBI]

Approximately 83 (40 + 43) kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.