CD45 Protein, Mouse (HEK293, Fc)

The CD45 protein is a multifunctional member of the protein tyrosine phosphatase family with heparan sulfate proteoglycan and heparin binding activity as well as protein tyrosine phosphatase activity. It plays a crucial role in lymphocyte differentiation, macromolecular metabolic processes, and signal transduction regulation. CD45 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD45 protein, expressed by HEK293 , with C-hFc labeled tag.

CD45 protein, a member of the protein tyrosine phosphatase family, exhibits versatile functions, including heparan sulfate proteoglycan binding activity, heparin binding activity, and protein tyrosine phosphatase activity. It is intricately involved in key processes such as lymphocyte differentiation, positive regulation of macromolecule metabolic processes, and regulation of signal transduction. CD45 acts upstream in lymphocyte-related pathways, including differentiation and activation, while being prominently located in the external side of the plasma membrane, focal adhesion, and membrane raft. Widely expressed in various structures such as the 3rd branchial arch, alimentary system, cardiovascular system, hemolymphoid system, and placenta, CD45's significance is underscored by its association with conditions like systemic lupus erythematosus. The human ortholog of this gene, PTPRC (protein tyrosine phosphatase receptor type C), is implicated in hepatitis C, multiple sclerosis, and severe combined immunodeficiency, highlighting its pivotal role in immune-related disorders.

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD45 is coated at 0.5 μg/mL (100 μL/well) can bind Recombinant Mouse Galectin-1 (HY-P78301). The ED₅₀ for this effect is 1.7 μg/mL.

Mouse

HEK293

C-hFc

NP_035340.3 (Q26-N425)

19264  [NCBI]

QTPTPSDGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFN

Approximately 130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.