CD5L Protein, Mouse (HEK293, His)

The CD5L protein is primarily expressed by macrophages and regulates inflammatory responses in infection or atherosclerosis. CD5L is incorporated into adipocytes via CD36-mediated endocytosis, thereby inhibiting lipid droplet size and affecting lipolysis. CD5L Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD5L protein, expressed by HEK293 , with C-6*His labeled tag.

CD5L Protein serves as a pivotal regulator of lipid synthesis, predominantly expressed by macrophages in lymphoid and inflamed tissues, orchestrating inflammatory responses in conditions such as infection or atherosclerosis. Its impact extends to adipocytes, where it inhibits lipid droplet size and influences lipolysis following incorporation into mature adipocytes through CD36-mediated endocytosis. By associating with cytosolic FASN, CD5L suppresses fatty acid synthase activity, resulting in the breakdown of triacylglycerols into glycerol and free fatty acids. In the context of obesity, CD5L-induced lipolysis contributes to inflammation and insulin resistance by recruiting inflammatory macrophages into adipose tissues. Additionally, CD5L exerts regulatory control over metabolic pathways in T-helper Th17 cells, influencing the expression of pro-inflammatory genes by modulating lipid content and limiting the synthesis of cholesterol ligands for RORC, the master transcription factor of Th17-cell differentiation. Notably present in non-pathogenic Th17 cells, CD5L alters polyunsaturated fatty acyl content, affecting metabolic proteins and consequently limiting RORC activity. Moreover, CD5L plays a role in obesity-associated autoimmunity by associating with IgM, interfering with Fcalpha/mu receptor binding, and enhancing the development of long-lived plasma cells producing high-affinity IgG autoantibodies. Beyond its metabolic functions, CD5L acts as an apoptosis inhibitor in macrophages, promoting their survival against the apoptotic effects of oxidized lipids in atherosclerosis. Furthermore, it contributes to the early response to microbial infection, serving as a pattern recognition receptor and promoting autophagy. CD5L's multifaceted roles underscore its significance in diverse physiological processes and its potential implications in various pathological conditions.

Mouse

HEK293

C-6*His

Q9QWK4 (E22-V352)

11801  [NCBI]

ESPTKVQLVGGAHRCEGRVEVEHNGQWGTVCDDGWDRRDVAVVCRELNCGAVIQTPRGASYQPPASEQRVLIQGVDCNGTEDTLAQCELNYDVFDCSHEEDAGAQCENPDSDLLFIPEDVRLVDGPGHCQGRVEVLHQSQWSTVCKAGWNLQVSKVVCRQLGCGRALLTYGSCNKNTQGKGPIWMGKMSCSGQEANLRSCLLSRLENNCTHGEDTWMECEDPFELKLVGGDTPCSGRLEVLHKGSWGSVCDDNWGEKEDQVVCKQLGCGKSLHPSPKTRKIYGPGAGRIWLDDVNCSGKEQSLEFCRHRLWGYHDCTHKEDVEVICTDFDV

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.