CD64 Protein, Rabbit (HEK293, His)

The CD64 protein is a high-affinity receptor for the immunoglobulin gamma Fc region and plays a key role in innate and adaptive immune responses. It prevents FCGR1A degradation by interacting with FCERG1 and FLNA to form a functional signaling complex. CD64 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived CD64 protein, expressed by HEK293 , with C-His labeled tag.

CD64 Protein emerges as a high-affinity receptor specifically designed for the Fc region of immunoglobulins gamma, playing a pivotal role in both innate and adaptive immune responses. Its dynamic functionality extends to forming a functional signaling complex through interaction with FCERG1. Additionally, CD64 interacts with FLNA, preventing FCGR1A degradation, and engages with EPB41L2, LAT, and PPL in intricate cellular processes. Furthermore, this receptor establishes connections with HCK and LYN, showcasing its versatility and involvement in various signaling cascades critical for immune system modulation.

Measured by its binding ability in a functional ELISA. Immobilized Rabbit CD64 at 1μg/mL (100μL/well) can bind biotinylated Rabbit IgG. The ED₅₀ for this effect is 1.224 μg/mL.

Rabbit

HEK293

C-6*His

XP_008262732.1 (Q16-F291)

100358696  [NCBI]

MWLWTALLLWVPVGGQEDPTKAVITLQPPWVDVFQEEAVTLQCEGPRQPGDSSAQWFLNGTAIRTLTPRYYITGAQANDSGEYRCQTELSAPSEPVQLQAHRDWLLLQVSGRVFTEGEPLTMRCHAWKNKIVYNVLFYQNGKAFKFSSQDSELTIPKTNVNHNGIYHCSAMGRYRYTSAGVSVTVQELFRVPVLRASSPLPLQEGSAVTLSCETKLLPDSPALRLYFSFYVGNKTVQARNVSSQYQIPRVGGEDLGLYWCEAATEDGGVRKCSVVLELGVLGSQAPNPVWF

Approximately 40-47 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.