CD64 Protein, Rat (HEK293, His)

CD64 Protein, with leukotriene receptor binding, crucially regulates processes like phagocytosis and pain perception, responding to epinephrine. Located in membrane rafts, CD64, encoded by FCGR1A, shows biased expression, especially in Spleen (RPKM 177.3) and Lung (RPKM 93.7). This emphasizes its importance in immune responses and sensory functions across diverse physiological contexts. CD64 Protein, Rat (HEK293, His) is the recombinant rat-derived CD64 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD64 Protein, Rat (HEK293, His) is 269 a.a., with molecular weight of 40-42 kDa.

CD64 Protein, characterized by its leukotriene receptor binding activity, plays a crucial role in various biological processes, including the positive regulation of phagocytosis and the sensory perception of pain, as well as the response to epinephrine. Situated in membrane rafts, CD64, encoded by the FCGR1A gene, exhibits a biased expression pattern with notable levels in the Spleen (RPKM 177.3), Lung (RPKM 93.7), and nine other tissues. This underlines its significance in immune responses and sensory functions across diverse physiological contexts.

Measured by its binding ability in a functional ELISA. Immobilized Rat CD64-his at 10 μg/mL (100 μL/well) can bind biotinylated human IgG1. The ED₅₀ for this effect is 0.0754 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rat CD64-his at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1,The ED₅₀ for this effect is 0.0754 μg/mL.

Rat

HEK293

C-His

NP_001094306 (V17-P285)

295279  [NCBI]

VVNATKAVVTLQPPWLSIFQEENVTLLCEGPHLGDSSTKWFINSTAIQISTPTYSILKASFKDSGEYKCQTGLSMPSDPVQLEIYRDWLLLQTSHRVLTEGEPLALRCHPWQNKKVYNVAFYRNGESLDFSQGSEVTILKTNLSHSGIYHCSAMGKKLFKSAGVSVTVKELFATPVLRVSLSSPFPEGSLVILNCETRLFLQSPGLRLYFSFYVGSKILEDRSTSSEYHIPRAEREDDGSYWCEVATEDGRVLKRSTKLELFGPQSSDP

40-42 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.