CD68 Protein, Mouse (sf9, His)

The CD68 protein plays a crucial role in the phagocytic activity of tissue macrophages, affecting lysosomal metabolism and interactions with cells and pathogens. It binds to specific lectins or selectins and guides macrophages to migrate to specific sites. CD68 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD68 protein, expressed by Sf9 insect cells , with C-His labeled tag.

The CD68 protein is implicated in potentially playing a crucial role in the phagocytic activities of tissue macrophages, influencing both intracellular lysosomal metabolism and extracellular interactions involving cell-cell and cell-pathogen encounters. It exhibits binding capabilities to tissue- and organ-specific lectins or selectins, facilitating the homing of distinct macrophage subsets to specific anatomical sites. The rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane suggests a dynamic mechanism that enables macrophages to navigate and traverse selectin-bearing substrates or interact with other cells. This multifaceted functionality of CD68 underscores its significance in the intricate processes of immune response and cellular interactions within diverse tissue microenvironments.

Mouse

Sf9 insect cells

C-His

P31996 (D21-S291)

12514  [NCBI]

Approximately 30.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.