CD73/5'-Nucleotidase Protein, Cynomolgus (HEK293, His)

CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His) is a CD73 protein with a His-flag. CD73/5'-Nucleotidase Protein a zinc-dependent, 70 kDa homodimeric enzyme that binds to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor^[1].

CD73/5'-Nucleotidase is a zinc-dependent, 70 kDa homodimeric enzyme bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor
. Vertebrate ecto-5'-nucleotidase (eN, EC 3.1.3.5) hydrolyzes ribo- and deoxyribonucleoside 5′-monophosphates including AMP, CMP, UMP, IMP, and GMP, whereby AMP generally is the most effectively hydrolyzed nucleotide
. CD73 is thought to function as a co-signaling molecule on T lymphocytes and an adhesion molecule that is required for lymphocyte binding to endothelium^[1].^[2]

Cynomolgus

HEK293

C-6*His

G7P3J1 (W27-K547)

/

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFETDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEIYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

58-80 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Herbert Zimmermann, ET AL. Cellular function and molecular structure of ecto-nucleotidases. Purinergic Signal. 2012 Sep;8(3):437-502.  [Content Brief]

  [2]. Dachuan Jin, et al. CD73 on tumor cells impairs antitumor T-cell responses: a novel mechanism of tumor-induced immune suppression. Cancer Res. 2010 Mar 15;70(6):2245-55.  [Content Brief]