CD8A-CD8B Heterodimer Protein, Mouse (HEK293, Flag-His)

The CD8A, a vital immune glycoprotein, acts as a coreceptor for MHC class I:peptide complexes, connecting T-cells to antigens presented by APCs. Its interaction with TCR and MHC class I recruits LCK kinase, initiating diverse signaling pathways. This mechanism enables CTLs to recognize and eliminate infected and tumor cells. In NK-cells, CD8A homodimers provide a survival mechanism for conjugation and lysis of target cells, contributing to lymphocyte survival and differentiation into memory CD8 T-cells. The heterodimer forms disulfide-linked complexes and homodimers, interacting with HLA-A/B2M and LCK in a zinc-dependent manner. CD8A-CD8B Heterodimer Protein, Mouse (HEK293, Flag-His) is a recombinant protein dimer complex containing mouse-derived CD8A-CD8B Heterodimer protein, expressed by HEK293, with C-His, C-Flag labeled tag. CD8A-CD8B Heterodimer Protein, Mouse (HEK293, Flag-His), has molecular weight of ~45.9 (23.7+22.2) kDa.

The CD8A, an integral membrane glycoprotein, plays a critical role in the immune response, fulfilling multiple functions in responses against both external and internal threats. In T-cells, it primarily functions as a coreceptor for the MHC class I molecule:peptide complex, interacting simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen-presenting cells (APCs). This interaction leads to the recruitment of the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK, in turn, initiates diverse intracellular signaling pathways, phosphorylating various substrates and ultimately promoting lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing the conjugation and lysis of multiple target cells. CD8A homodimer molecules also contribute to the survival and differentiation of activated lymphocytes into memory CD8 T-cells. The CD8A forms disulfide-linked complexes at the cell surface and also homodimers in various cell types, including NK-cells and peripheral blood T-lymphocytes. It interacts with the MHC class I HLA-A/B2M dimer and with LCK in a zinc-dependent manner.

Mouse

HEK293

C-His;C-Flag

P01731 (K28-Y196)&P10300 (L22-T175)

12525  [NCBI]&12526  [NCBI]

Approximately 45.9 (23.7+22.2) kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.