2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2) Serine/Threonine Kinase Proteins
  5. Cyclin-Dependent Kinases (CDKs)
  6. Cyclin Dependent Kinase 1 (CDK1)
  7. CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag)

CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag)

Cat. No.: HY-P702674
Handling Instructions

The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK1-CCNE2, expressed by Sf9 insect cells , with N-His, N-GST, N-Flag labeled tag. ,

For research use only. We do not sell to patients.

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Description

The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK1-CCNE2, expressed by Sf9 insect cells , with N-His, N-GST, N-Flag labeled tag. ,
Background

The CDK1/CDC2-cyclin-B complex is a key regulator of the cell cycle. It plays an important control role in the interphase fertilized egg and is crucial for the early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates the CDK1/cyclin complex. This complex phosphorylates multiple substrates, triggering centrosome dissociation, Golgi dynamics, nuclear envelope disruption, and chromosome condensation. After chromosomes are condensed and aligned on the metaphase plate, CDK1 activity is turned off by WEE1- and PKMYT1-mediated phosphorylation, allowing sister chromatid separation, chromosome decondensation, nuclear envelope remodeling, and cytokinesis. When DNA is damaged, CDK1 will be inactivated by phosphorylation mediated by PKR/EIF2AK2 and WEE1, thereby stopping the cell cycle and genome replication and promoting DNA repair. After repair is completed, CDK1 is reactivated by WIP1-dependent signaling to resume cell cycle progression. In proliferating cells, CDK1-mediated phosphorylation can inhibit FOXO1 interactions and promote FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. In addition, phosphorylation of CDK1 can also regulate microtubule dynamics, spindle formation and other processes.
Species

Human
Source

Sf9 insect cells
Tag

N-His;N-GST;N-Flag
Accession

P06493-1 (M1-M297)&O96020-1 (S2-H404 )
Gene ID

983&9134
Synonyms
CDK1; cyclin-dependent kinase 1; CDC2, cell division cycle 2, G1 to S and G2 to M; CDC28A; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; CDC2; P34CDC2; MGC111195; DKFZp686L20222
Molecular Weight

60.7 kDa&49.4 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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