CDK16-CCNY Protein, Human (sf9, GST, Flag)

PCTAIRE1 protein is a multifunctional kinase that plays a key role in vesicle-mediated transport and regulation of GH1 release, emphasizing neuroendocrine function. Its involvement includes NSF phosphorylation, influencing vesicle dynamics, and important contributions to spermatogenesis and reproductive processes. CDK16-CCNY Protein, Human (sf9, GST, Flag) is the recombinant human-derived CDK16-CCNY, expressed by Sf9 insect cells , with N-GST, N-Flag labeled tag. ,

PCTAIRE1 protein, a versatile kinase, assumes a pivotal role in diverse cellular processes, particularly in vesicle-mediated transport and exocytosis. Its involvement extends to the regulation of GH1 release by brain neurons, underlining its significance in neuroendocrine functions. PCTAIRE1's reach encompasses the phosphorylation of NSF, modulating NSF oligomerization and thereby influencing crucial aspects of vesicle dynamics. Notably, it emerges as an essential player in spermatogenesis, contributing to normal reproductive processes. Additionally, PCTAIRE1 plays a key role in the intricate regulation of neuron differentiation and dendrite development, emphasizing its impact on neuronal morphology. Beyond its neuronal functions, PCTAIRE1 is implicated in the regulation of insulin secretion in response to changes in blood glucose levels, implicating its role in metabolic homeostasis. The kinase's ability to phosphorylate CCNY at 'Ser-336' in vitro further highlights its diverse molecular interactions and underscores its multifaceted role in cellular signaling.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of CDK16-CCNY for its substrate is 20 μM.

Human

Sf9 insect cells

N-GST;N-Flag

Q00536-1 (M1-F496)&Q8ND76-1 (M1-S341)

5127&219771

82.3 kDa & 41.3 kDa

Greater than 80 % as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 5% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.