CDK3 Protein, Human (Sf9, GST)

CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3 Protein, Human (Sf9, GST) is the recombinant human-derived CDK3, expressed by Sf9 insect cells , with GST labeled tag. The total length of CDK3 Protein, Human (Sf9, GST) is 305 a.a.,

CDK3 Protein is a serine/threonine-protein kinase that assumes a pivotal role in the regulation of the eukaryotic cell cycle, specifically influencing G0-G1 and G1-S transitions. During interphase, it interacts with CCNC/cyclin-C. The kinase phosphorylates key substrates, including histone H1, ATF1, RB1, and CABLES1. ATF1 phosphorylation induced by CDK3-CCNE1 triggers ATF1 transactivation and transcriptional activities, consequently fostering cell proliferation and transformation. Moreover, the CDK3/cyclin-C-mediated phosphorylation of RB1 is essential for the G0-G1 transition. Notably, CDK3-CCNE1 contributes to the G1-S transition, potentially by participating in the activation of E2F1, E2F2, and E2F3 in a RB1-independent manner. This intricate interplay positions CDK3-CCNE1 as a crucial orchestrator of cell cycle progression.

Human

Sf9 insect cells

GST

Q00526 (M1-H305)

1018

Approximately 61.6 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.