1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. CEA Cell Adhesion Molecule 19 (CEACAM19)
  6. CEACAM19 Protein, Human (HEK293, His)

CEACAM19 Protein, Human (HEK293, His)

Cat. No.: HY-P76816
SDS COA Handling Instructions

CEACAM19 Protein, Human (HEK293, His) is the recombinant human-derived CEACAM19 protein, expressed by HEK293 , with C-His labeled tag. The total length of CEACAM19 Protein, Human (HEK293, His) is 157 a.a., with molecular weight of ~15.4 KDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CEACAM19 Protein, Human (HEK293, His) is the recombinant human-derived CEACAM19 protein, expressed by HEK293 , with C-His labeled tag. The total length of CEACAM19 Protein, Human (HEK293, His) is 157 a.a., with molecular weight of ~15.4 KDa.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of the L929 cells. The ED50 for this effect is 0.5232 μg/mL, corresponding to a specific activity is 1.911×103 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of the L929 cells. The ED50 for this effect is 0.5232 μg/mL, corresponding to a specific activity is 1.911×103 units/mg.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q7Z692-1 (A33-G157)

Gene ID

56971  [NCBI]

Molecular Construction
N-term
CEACAM19 (A33-G157)
Accession # Q7Z692-1
His
C-term
Synonyms
Carcinoembryonic antigen-related cell adhesion molecule 19; CEAL1
AA Sequence

ALYIQKIPEQPQKNQDLLLSVQGVPDTFQDFNWYLGEETYGGTRLFTYIPGIQRPQRDGSAMGQRDIVGFPNGSMLLRRAQPTDSGTYQVAITINSEWTMKAKTEVQVAEKNKELPSTHLPTNAG

Molecular Weight

Approximately 18-24 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CEACAM19 Protein, Human (HEK293, His)
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HY-P76816
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