CES2/carboxylesterase 2 Protein, Human (HEK293,C-His,Solution)

CES2 Protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It exhibits high catalytic efficiency, particularly in the hydrolysis of substances like cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 participates in the hydrolysis of aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. The protein is involved in the conversion of monoacylglycerides to free fatty acids and glycerol and contributes to the hydrolysis of 2-arachidonoylglycerol and prostaglandins. CES2/carboxylesterase 2 Protein, Human (HEK293,C-His,Solution) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of CES2/carboxylesterase 2 Protein, Human (HEK293,C-His,Solution) is 533 a.a., with molecular weight of ~50-70 kDa.

The CES2/Carboxylesterase 2 Protein plays a crucial role in the detoxification of xenobiotics and the activation of ester and amide prodrugs. It exhibits high catalytic efficiency in the hydrolysis of various compounds, including cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 hydrolyzes aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. Notably, it is involved in the conversion of monoacylglycerides to free fatty acids and glycerol. Furthermore, CES2 demonstrates activity in the hydrolysis of 2-arachidonoylglycerol and prostaglandins, highlighting its versatility in enzymatic functions with potential implications in various biological processes and drug metabolism.

Measured by its ability to hydrolyze p-nitrophenylacetate.Which can be measured by absorbance at 410 nm. The specific activity is 42718.666 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-10*His

O00748-1 (Q27-L559)

8824

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Approximately 50-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.5.

<1 EU/μg, determined by LAL method

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.