CES2/carboxylesterase 2 Protein, Human (HEK293,C-His)

The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag. The total length of CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) is 533 a.a., with molecular weight of ~60.4 KDa.

The CES2/Carboxylesterase 2 Protein plays a crucial role in the detoxification of xenobiotics and the activation of ester and amide prodrugs. It exhibits high catalytic efficiency in the hydrolysis of various compounds, including cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 hydrolyzes aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. Notably, it is involved in the conversion of monoacylglycerides to free fatty acids and glycerol. Furthermore, CES2 demonstrates activity in the hydrolysis of 2-arachidonoylglycerol and prostaglandins, highlighting its versatility in enzymatic functions with potential implications in various biological processes and drug metabolism.

Measured by its ability to hydrolyze p-nitrophenylacetate.The specific activity is >20000 pmols/min/μg.

Human

HEK293

C-His

O00748-1 (Q27-L559)

8824  [NCBI]

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Approximately 60.4 kDa.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.