CFHR5 Protein, Human (HEK293, His)

CFHR5 is a first-line complement regulator that is actively involved in the complex dynamics of the regulatory system. Dimeric CFHR5 has significant affinity for tissue-bound complement fragments, thereby challenging CFH. CFHR5 Protein, Human (HEK293, His) is the recombinant human-derived CFHR5 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CFHR5 Protein, Human (HEK293, His) is 551 a.a., with molecular weight of ~61.0 kDa.

CFHR5 stands at the forefront of complement regulation, actively participating in the intricate dynamics of this regulatory system. In its dimerized forms, CFHR5 demonstrates a notable affinity for tissue-bound complement fragments, effectively challenging the physiological complement inhibitor CFH. This versatile regulator engages in a head-to-tail homodimeric configuration and forms heterodimers with CFHR1 or CFHR2, underlining its adaptable role in orchestrating complement-related processes. Moreover, CFHR5 exhibits the capacity to bind C3b in vitro, adding an additional layer to its repertoire of interactions and highlighting its intricate involvement in the regulation of complement pathways.

Human

HEK293

C-6*His

Q9BXR6 (E19-E569)

81494  [NCBI]

EGTLCDFPKIHHGFLYDEEDYNPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCTEEGWSPTPKCLRMCSFPFVKNGHSESSGLIHLEGDTVQIICNTGYSLQNNEKNISCVERGWSTPPICSFTKGECHVPILEANVDAQPKKESYKVGDVLKFSCRKNLIRVGSDSVQCYQFGWSPNFPTCKGQVRSCGPPPQLSNGEVKEIRKEEYGHNEVVEYDCNPNFIINGPKKIQCVDGEWTTLPTCVEQVKTCGYIPELEYGYVQPSVPPYQHGVSVEVNCRNEYAMIGNNMITCINGIWTELPMCVATHQLKRCKIAGVNIKTLLKLSGKEFNHNSRIRYRCSDIFRYRHSVCINGKWNPEVDCTEKREQFCPPPPQIPNAQNMTTTVNYQDGEKVAVLCKENYLLPEAKEIVCKDGRWQSLPRCVESTAYCGPPPSINNGDTTSFPLSVYPPGSTVTYRCQSFYKLQGSVTVTCRNKQWSEPPRCLDPCVVSEENMNKNNIQLKWRNDGKLYAKTGDAVEFQCKFPHKAMISSPPFRAICQEGKFEYPICE

Approximately 61.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.