CHIT1/Chitotriosidase-1 Protein, Human (HEK293, His)

CHIT1/Chitotriosidase-1 protein, an enzyme degrading chitin, chitotriose, and chitobiose, potentially defends against nematodes and pathogens. Isoform 3 lacks enzymatic activity, highlighting the complexity of CHIT1 variants. The ability of CHIT1 to break down chitin suggests involvement in immune defense mechanisms. The existence of enzymatically inactive isoform 3 hints at diverse functions or regulatory roles within biological processes. CHIT1/Chitotriosidase-1 Protein, Human (HEK293, His) is the recombinant human-derived CHIT1/Chitotriosidase-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of CHIT1/Chitotriosidase-1 Protein, Human (HEK293, His) is 445 a.a., with molecular weight of ~48 KDa.

The CHIT1/Chitotriosidase-1 protein serves as an enzyme capable of degrading chitin, chitotriose, and chitobiose, indicating its potential role in the defense against nematodes and other pathogens. However, it's noteworthy that isoform 3 of CHIT1 lacks enzymatic activity. The ability of CHIT1 to break down chitin-containing substances suggests its involvement in immune defense mechanisms, particularly against certain types of pathogens. The existence of isoform 3, which lacks enzymatic activity, underscores the complexity of CHIT1 protein variants and hints at potential diverse functions or regulatory roles within biological processes.

Measured by its ability to cleave 4-Methylumbelliferyl-beta-D-N,N’,N”-triacetylchitotriose (4-MU-TACT) that incubate at room temperature for 5 minutes. The specific activity is 2707 pmol/min/µg.

Human

HEK293

C-His

Q13231 (A22-N466)

1118  [NCBI]

Approximately 48 kDa.

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 12.5 mM Tris, 75 mM NaCl, 50% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.