Chondroitinase ABC Protein, P. vulgaris (His)

Chondroitinase ABC protein is an endolytic glycosaminoglycan lyase with broad specificity that effectively degrades polysaccharides such as chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, and smaller degree of hyaluronic acid. This enzyme activity is facilitated by β-elimination of the 1,4-hexosamine bond, resulting in unsaturated tetra- and disaccharides. Chondroitinase ABC Protein, P. vulgaris (His) is the recombinant Chondroitinase ABC protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of Chondroitinase ABC Protein, P. vulgaris (His) is 997 a.a., with molecular weight of ~110 kDa.

Chondroitinase ABC protein is an endolytic glycosaminoglycan lyase with broad specificity, capable of degrading various polysaccharides, including chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, and, to a lesser extent, hyaluronan. This enzyme operates through beta-elimination of the 1,4-hexosaminidic bond, producing unsaturated tetrasaccharides and disaccharides. Importantly, Chondroitinase ABC does not exhibit activity against keratan sulfate, heparan sulfate, and heparin. Its ability to disrupt the normal organization of the extracellular matrix (ECM) positions it as a key player in promoting functional recovery in the injured central nervous system (CNS). This underscores its potential therapeutic significance in facilitating neural regeneration and repair through targeted modification of specific glycosaminoglycans within the ECM. (

Measured by its ability to hydrolyze chondroitin sulfate. The specific activity is >15,000 pmol/min/μg.

Others

E. coli

N-His;N-6*His

P59807 (A25-P1021)

2827933  [NCBI]

ATSNPAFDPKNLMQSEIYHFAQNNPLADFSSDKNSILTLSDKRSIMGNQSLLWKWKGGSSFTLHKKLIVPTDKEASKAWGRSSTPVFSFWLYNEKPIDGYLTIDFGEKLISTSEAQAGFKVKLDFTGWRAVGVSLNNDLENREMTLNATNTSSDGTQDSIGRSLGAKVDSIRFKAPSNVSQGEIYIDRIMFSVDDARYQWSDYQVKTRLSEPEIQFHNVKPQLPVTPENLAAIDLIRQRLINEFVGGEKETNLALEENISKLKSDFDALNIHTLANGGTQGRHLITDKQIIIYQPENLNSQDKQLFDNYVILGNYTTLMFNISRAYVLEKDPTQKAQLKQMYLLMTKHLLDQGFVKGSALVTTHHWGYSSRWWYISTLLMSDALKEANLQTQVYDSLLWYSREFKSSFDMKVSADSSDLDYFNTLSRQHLALLLLEPDDQKRINLVNTFSHYITGALTQVPPGGKDGLRPDGTAWRHEGNYPGYSFPAFKNASQLIYLLRDTPFSVGESGWNNLKKAMVSAWIYSNPEVGLPLAGRHPFNSPSLKSVAQGYYWLAMSAKSSPDKTLASIYLAISDKTQNESTAIFGETITPASLPQGFYAFNGGAFGIHRWQDKMVTLKAYNTNVWSSEIYNKDNRYGRYQSHGVAQIVSNGSQLSQGYQQEGWDWNRMQGATTIHLPLKDLDSPKPHTLMQRGERGFSGTSSLEGQYGMMAFDLIYPANLERFDPNFTAKKSVLAADNHLIFIGSNINSSDKNKNVETTLFQHAITPTLNTLWINGQKIENMPYQTTLQQGDWLIDSNGNGYLITQAEKVNVSRQHQVSAENKNRQPTEGNFSSAWIDHSTRPKDASYEYMVFLDATPEKMGEMAQKFRENNGLYQVLRKDKDVHIILDKLSNVTGYAFYQPASIEDKWIKKVNKPAIVMTHRQKDTLIVSAVTPDLNMTRQKAATPVTINVTINGKWQSADKNSEVKYQVSGDNTELTFTSYFGIPQEIKLSPLP

Approximately110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.