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CIAO1 Protein, Human (His, Strep)

Cat. No.: HY-P702047
Handling Instructions

CIAO1 protein is a key player in the cytosolic iron-sulfur protein assembly (CIA) complex, interacting with CIAO2A or CIAO2B and MMS19 to affect different iron-sulfur protein assembly pathways. The CIAO1:CIAO2B:MMS19 assembly facilitates most cytoplasmic and nuclear Fe/S proteins, while the CIAO1:CIAO2A complex contributes to ACO1 maturation and IREB2 stabilization. CIAO1 Protein, Human (His, Strep) is the recombinant human-derived CIAO1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of CIAO1 Protein, Human (His, Strep) is 339 a.a., .

For research use only. We do not sell to patients.

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Description

CIAO1 protein is a key player in the cytosolic iron-sulfur protein assembly (CIA) complex, interacting with CIAO2A or CIAO2B and MMS19 to affect different iron-sulfur protein assembly pathways. The CIAO1:CIAO2B:MMS19 assembly facilitates most cytoplasmic and nuclear Fe/S proteins, while the CIAO1:CIAO2A complex contributes to ACO1 maturation and IREB2 stabilization. CIAO1 Protein, Human (His, Strep) is the recombinant human-derived CIAO1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of CIAO1 Protein, Human (His, Strep) is 339 a.a., .

Background

CIAO1 emerges as a pivotal player in the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein entity orchestrating the integration of iron-sulfur clusters into extramitochondrial Fe/S proteins. Its dynamic role within this complex involves specific interactions with CIAO2A or CIAO2B and MMS19, influencing distinct branches of iron-sulfur protein assembly depending on its binding partners. The CIAO1:CIAO2B:MMS19 assembly is particularly involved in facilitating the assembly of most cytosolic-nuclear Fe/S proteins, while the CIAO1:CIAO2A complex specifically contributes to the maturation of ACO1 and stabilization of IREB2. Beyond its involvement in iron-sulfur biogenesis, CIAO1 extends its influence to modulating the transactivation activity of WT1 and participating in chromosome segregation through its association with the mitotic spindle-associated MMXD complex. As an integral component of the CIA complex and various interacting networks, CIAO1 stands as a versatile orchestrator in cellular processes involving iron-sulfur clusters and beyond.

Species

Human

Source

E. coli

Tag

N-Strep;N-6*His

Accession

O76071 (M1-L339)

Gene ID

9391

Molecular Construction
N-term
Strep-6*His
CIAO1 (M1-L339)
Accession # O76071
C-term
Synonyms
CIAO1; Probable cytosolic iron-sulfur protein assembly protein CIAO1; WD repeat-containing protein 39
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CIAO1 Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CIAO1 Protein, Human (His, Strep)
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HY-P702047
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