CKAP1/TBCB Protein, Human (His)

CKAP1/TBCB protein plays a pivotal role in the tubulin folding pathway, binding to alpha-tubulin folding intermediates after their chaperonin interaction, crucial for tubulin heterodimer transition. It regulates heterodimer dissociation, potentially acting as a negative regulator of axonal growth. The supercomplex, formed with cofactors A to E, captures and stabilizes tubulin, releasing committed polypeptides to the native state. CKAP1/TBCB's interactions with GAN and DCTN1 extend its functional roles in cellular processes. CKAP1/TBCB Protein, Human (His) is the recombinant human-derived CKAP1/TBCB protein, expressed by E. coli, with N-His labeled tag. The total length of CKAP1/TBCB Protein, Human (His) is 244 a.a., with molecular weight of 33-37 kDa.

The CKAP1/TBCB protein exhibits a multifaceted role in the tubulin folding pathway, binding to alpha-tubulin folding intermediates subsequent to their interaction with cytosolic chaperonin, a pivotal step in the transition from newly synthesized tubulin to properly folded heterodimer. It plays a crucial role in regulating tubulin heterodimer dissociation and may function as a negative regulator of axonal growth. The formation of a supercomplex composed of cofactors A to E is integral to its mechanism. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation, while cofactor E binds to the cofactor D-tubulin complex. The subsequent interaction with cofactor C leads to the release of tubulin polypeptides committed to the native state. Additionally, cofactors B and E can form a heterodimer that binds to alpha-tubulin, enhancing their capacity to dissociate tubulin heterodimers. CKAP1/TBCB also interacts with GAN and DCTN1, further expanding its functional associations in cellular processes.

Human

E. coli

N-His

Q99426 (M1-I244)

1155  [NCBI]

33-37 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.