1. Recombinant Proteins
  2. Receptor Proteins
  3. Pattern Recognition Receptors
  4. C-type Lectin Receptors
  5. CLEC14A
  6. CLEC14A Protein, Mouse (HEK293, His)

CLEC14A Protein, Mouse (HEK293, His)

Cat. No.: HY-P76828
Data Sheet SDS COA Handling Instructions

CLEC14A Protein, Mouse (HEK293, His) is the recombinant mouse-derived CLEC14A protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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5 μg USD 60 In-stock
10 μg USD 95 In-stock
50 μg USD 250 In-stock
100 μg USD 425 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CLEC14A Protein, Mouse (HEK293, His) is the recombinant mouse-derived CLEC14A protein, expressed by HEK293 , with C-His labeled tag.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CLEC14A Protein is immobilized at 1 µg/mL (100 µL/well) can bind Mouse VEGF R3 Protein. The ED50 for this effect is 1.61 μg/mL.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q8VCP9 (E22-T386)

Gene ID
Molecular Construction
N-term
CLEC14A (E22-T386)
Accession # Q8VCP9
His
C-term
Synonyms
C-type lectin domain family 14 member A; EGFR-5; C14orf27
AA Sequence

EHPTADRAACSASGACYSLHHATFKRRAAEEACSLRGGTLSTVHSGSEFQAVLLLLRAGPGPGGGSKDLLFWVALERSISQCTQEKEPLRGFSWLHPDSEDSEDSPLPWVEEPQRSCTVRKCAALQATRGVEPAGWKEMRCHLRTDGYLCKYQFEVLCPAPRPGAASNLSFQAPFRLSSSALDFSPPGTEVSAMCPGDLSVSSTCIQEETSAHWDGLFPGTVLCPCSGRYLLAGKCVELPDCLDHLGDFTCECAVGFELGKDGRSCETKVEEQLTLEGTKLPTRNVTATPAGAVTNRTWPGQVYDKPGEMPQVTEILQWGTQSTLPTIQKTPQTKPKVTGTPSGSVVLNYTSSPPVSLTFDTSST

Molecular Weight

Approximately 70-80 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CLEC14A Protein, Mouse (HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CLEC14A Protein, Mouse (HEK293, His)
Cat. No.:
HY-P76828
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