CMKLR1 Protein, Human (Cell-Free, His)

CMKLR1 protein is a receptor for chemerin/RARRES2 and resolvin E1, activating G protein and β-arrestin pathways when RARRES2 interacts. This triggers a cascade of reactions including calcium mobilization, MAP kinase phosphorylation, and PI3K activation, leading to various effects including reduced immune response, adipogenesis, and angiogenesis. CMKLR1 Protein, Human (Cell-Free, His) is the recombinant human-derived CMKLR1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CMKLR1 Protein, Human (Cell-Free, His) is 373 a.a., with molecular weight of 45.1 kDa.

The CMKLR1 Protein functions as a receptor for the chemoattractant adipokine chemerin/RARRES2 and the omega-3 fatty acid-derived molecule resolvin E1. Upon interaction with RARRES2, CMKLR1 activates G proteins G(i)/G(o) and beta-arrestin pathways, triggering cellular responses through second messenger pathways like intracellular calcium mobilization, phosphorylation of MAP kinases (MAPK1/MAPK3), TYRO3, MAPK14/P38MAPK, and PI3K. These cascades lead to multifunctional effects, including the reduction of immune responses, enhancement of adipogenesis, and angiogenesis. Resolvin E1, on the other hand, down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 also plays a positive regulatory role in adipogenesis and adipocyte metabolism. In the context of microbial infection, CMKLR1 acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR, and SIVSM62A) as well as for a primary HIV-1 strain (92UG024-2).

Human

E. coli Cell-free

N-10*His

Q99788 (M1-L373)

1240

MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMKKTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCISVLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMDPVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLNLLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERTSMNERETGML

45.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.