CNTN5/Contactin-5 Protein, Human (HEK293, His)

CNTN5/Contactin-5 protein mediates cell surface interactions in nervous system development, promoting neurite outgrowth in cerebral cortical neurons but not in hippocampal neurons. It is involved in auditory system neuronal activity and interacts with PTPRG, potentially regulating neuronal functions and connectivity. CNTN5/Contactin-5 Protein, Human (HEK293, His) is the recombinant human-derived CNTN5/Contactin-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of CNTN5/Contactin-5 Protein, Human (HEK293, His) is 1041 a.a., with molecular weight of ~115-150 kDa.

Contactin-5 (CNTN5) plays a crucial role in mediating cell surface interactions during nervous system development. While it exhibits neurite outgrowth-promoting activity in cerebral cortical neurons, it does not exert the same effect in hippocampal neurons. CNTN5 is implicated in neuronal activity within the auditory system, highlighting its potential role in sensory processes. Furthermore, it interacts with protein tyrosine phosphatase receptor gamma (PTPRG), suggesting a molecular partnership that may contribute to the regulation of neuronal functions and connectivity.

Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 1.986 μg/mL, corresponding to a specific activity is 503.525 units/mg.

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  Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 1.986 μg/mL, corresponding to a specific activity is 503.525 units/mg.

Human

HEK293

C-His

O94779 (E19-Q1059)

53942  [NCBI]

EYSKSLPGLSTSYAALLRIKKSSSSSLFGSKTRPRYSSPSLGTLSASSPSWLGAAQNYYSPINLYHSSDAFKQDESVDYGPVFVQEPDDIIFPTDSDEKKVALNCEVRGNPVPSYRWLRNGTEIDLESDYRYSLIDGTFIISNPSEAKDSGHYQCLATNTVGSILSREATLQFAYLGNFSGRTRSAVSVREGQGVVLMCSPPPHSPEIIYSWVFNEFPSFVAEDSRRFISQETGNLYISKVQTSDVGSYICLVKNTVTNARVLSPPTPLTLRNDGVMGEYEPKIEVHFPFTVTAAKGTTVKMECFALGNPVPTITWMKVNGYIPSKARLRKSQAVLEIPNVQLDDAGIYECRAENSRGKNSFRGQLQVYTYPHWVEKLNDTQLDSGSPLRWECKATGKPRPTYRWLKNGVPLSPQSRVEMVNGVLMIHNVNQSDAGMYQCLAENKYGAIYASAELKILASAPTFALNQLKKTIIVTKDQEVVIECKPQGSPKPTISWKKGDRAVRENKRIAILPDGSLRILNASKSDEGKYVCRGENVFGSAEIIASLSVKEPTRIELTPKRTELTVGESIVLNCKAIHDASLDVTFYWTLKGQPIDFEEEGGHFESIRAQASSADLMIRNILLMHAGRYGCRVQTTADSVSDEAELLVRGPPGPPGIVIVEEITESTATLSWSPAADNHSPISSYNLQARSPFSLGWQTVKTVPEIITGDMESAMAVDLNPWVEYEFRVVATNPIGTGDPSTPSRMIRTNEAVPKTAPTNVSGRSGRRHELVIAWEPVSEEFQNGEGFGYIVAFRPNGTRGWKEKMVTSSEASKFIYRDESVPPLTPFEVKVGVYNNKGDGPFSQIVVICSAEGEPSAAPTDVKATSVSVSEILVAWKHIKESLGRPQGFEVGYWKDMEQEDTAETVKTRGNESFVILTGLEGNTLYHFTVRAYNGAGYGPPSSEVSATTKKSPPSQAPSNLRWEQQGSQVSLGWEPVIPLANESEVVGYKVFYRQEGHSNSQVIETQKLQAVVPLPDAGVYIIEVRAYSEGGDGTASSQ

Approximately 115-150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.