Coagulation factor IX/F9 Protein, Human (HEK293, His)

Factor IX (F9) is a vitamin K-dependent plasma protein that plays a critical role in the intrinsic pathway of coagulation. Factor IX coordinates the conversion of factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Coagulation factor IX/F9 Protein, Human (HEK293, His) is the recombinant human-derived Coagulation factor IX/F9 protein, expressed by HEK293 , with C-6*His labeled tag.

Coagulation factor IX, also known as F9, is a vitamin K-dependent plasma protein crucial for blood coagulation, particularly in the intrinsic pathway. This protein plays a pivotal role in converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. As a key component of the coagulation cascade, factor IX contributes to the formation of a stable blood clot, preventing excessive bleeding. The described mechanism highlights the intricate interplay of factor IX with other coagulation factors and cofactors, underscoring its essential role in maintaining hemostasis.

1. Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read).The specific activity is >200 pmol/min/μg, as measured under the described conditions.
2. Measured by its ability to cleave the peptide substrate Z-D-Arg-Gly-Arg-pNA and the specific activity is >20 pmol/min/ug. (Activate human Coagulation factor IX wih Thermolysin in Assay Buffer, 37°C, 90 min.)

Human

HEK293

C-6*His

P00740-1 (T29-T461)

2158  [NCBI]

TVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.