Coagulation Factor X/F10 Protein, Human (HEK293, Fc)

Factor X (F10) is a vitamin K-dependent glycoprotein that plays a key role in the blood coagulation process as factor Xa. Factor Xa, along with factor Va, calcium, and phospholipids, coordinates the conversion of prothrombin to thrombin, a key step in the coagulation cascade. Coagulation Factor X/F10 Protein, Human (HEK293, Fc) is the recombinant human-derived Coagulation Factor X/F10 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Coagulation Factor X/F10 Protein, Human (HEK293, Fc) is 457 a.a., with molecular weight of 80-130 & 25 kDa, respectively.

Coagulation Factor X, also known as F10, is a vitamin K-dependent glycoprotein crucial for the blood clotting process. Factor Xa, an activated form of Factor X, plays a pivotal role in the coagulation cascade by converting prothrombin to thrombin in the presence of factor Va, calcium, and phospholipids. This enzymatic conversion is a key step in the formation of a stable blood clot, essential for preventing excessive bleeding. The interaction of Factor X with its cofactors and substrates underscores its significance in the intricate network of molecular events that regulate hemostasis.

Measured by its ability to cleave 20 µM fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 1174.20 pmol/min/µg.

Human

HEK293

C-hFc

P00742 (N32-K488)

2159  [NCBI]

NNILARVTRANSFLEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCKDGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK

80-130&25 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM MES,150 mM NaCl, 0.2 mM CaCl₂, pH 5.5 or pH 6.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.