Coagulation factor XIII A/F13A Protein, Human (HEK293, His)

Coagulation factor XIII A chain is the A chain of factor XIII. As a coagulation factor, Factor XIII A chain alters clot stabilization and bleeding. Factor XIII A chain regulates multiple aspects of wound healing: stabilizes fibrin clots and extracellular matrix, modulates monocyte functions, promote angiogenesis, and upregulates proangiogenic early growth response transcription factor 1^[1][2][3][4].
Coagulation factor XIII A/F13A Protein, Human (HEK293, His) is the recombinant human-derived Coagulation factor XIII A/F13A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Coagulation factor XIII A/F13A Protein, Human (HEK293, His) is 694 a.a., with molecular weight of 80-90 kDa.

Coagulation factor XIII is critical in the coagulation cascade chain, and enhances the stability of blood clot formation. Factor XIII is involved in trauma, wound healing, tissue repair, pregnancy, and even bone metabolism. Factor XIII can bind to αvβ3 integrin, inducing VEGFR-2 activation, promoting endothelial cell proliferation and survival, and angiogenesis^[1].
Factor XIII, a transglutaminase, is a complex of two A (gene F13A1) chains with catalytic activity and two B chains (gene F13B)^[2]. Factor XIII A chain can be activated by thrombin and Ca²⁺. Factor XIII A usually exists in the cytoplasm of certain cells, particularly platelets and monocytes/macrophages^[3].
Factor XIII A chain plays a role in wound healing, inflammatory and malignant diseases^[4].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH27963.1 (G39-M732)

2162  [NCBI]

GVNLQEFLNVTSVHLFKERWDTNKVDHHTDKYENNKLIVRRGQSFYVQIDFSRPYDPRRDLFRVEYVIGRYPQENKGTYIPVPIVSELQSGKWGAKIVMREDRSVRLSIQSSPKCIVGKFRMYVAVWTPYGVLRTSRNPETDTYILFNPWCEDDAVYLDNEKEREEYVLNDIGVIFYGEVNDIKTRSWSYGQFEDGILDTCLYVMDRAQMDLSGRGNPIKVSRVGSAMVNAKDDEGVLVGSWDNIYAYGVPPSAWTGSVDILLEYRSSENPVRYGQCWVFAGVFNTFLRCLGIPARIVTNYFSAHDNDANLQMDIFLEEDGNVNSKLTKDSVWNYHCWNEAWMTRPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVIVEFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM

80-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM NaCl, 5% Sucrose, 0.3% Histidine , pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.