Complement C3/C3a Protein, Bovine (His-SUMO)

Complement C3/C3a proteins play a key role in initiating the complement system, serving as substrates for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, yielding C3b, which is covalently bound to surface or immune aggregates. Complement C3/C3a Protein, Bovine (His-SUMO) is the recombinant bovine-derived Complement C3/C3a protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

C3 assumes a pivotal role in the initiation of the complement system, serving as a substrate for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, generating C3b, which can covalently bind to cell surface carbohydrates or immune aggregates through its reactive thioester moiety. Concurrently, the anaphylatoxin C3a is produced as a result of C3 degradation, functioning as a mediator in local inflammatory processes. C3a induces smooth muscle contraction, enhances vascular permeability, and stimulates histamine release from mast cells and basophilic leukocytes. Additionally, in chronic inflammation, C3a acts as a chemoattractant for neutrophils.

Bovine

E. coli

N-His;N-SUMO

Q2UVX4 (S747-Q953)

280677  [NCBI]

SDLDDDIIPEEDIISRSQFPESWLWTVIEDLKQADKNGISTKLMNVFLKDSITTWEILAVSLSDKKGICVADPYEVTVMQDFFIDLRLPYSVVRNEQVEIRAILYNYREAENLKVRVELLYNPAFCSLATAKKRHQQTITIPARSSVAVPYVIVPLKIGLHEVEVKAAVYNHFISDGVKKTLKVVPEGVRVNKTVAVRTLNPEHLGQ

Approximately 44 kDa.The reducing (R) protein migrat es as 44 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.