1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Contactin-1
  6. Contactin-1/CNTN1 Protein, Human (HEK293, His)

Contactin-1/CNTN1 Protein, Human (HEK293, His)

Cat. No.: HY-P74225
COA Handling Instructions

Contactin-1/CNTN1 proteins mediate cell surface interactions in the developing nervous system. Contactin-1/CNTN1 Protein, Human (HEK293, His) is the recombinant human-derived Contactin-1/CNTN1, expressed by HEK293 , with C-His labeled tag. The total length of Contactin-1/CNTN1 Protein, Human (HEK293, His) is 973 a.a.,

For research use only. We do not sell to patients.

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100 μg $890 In-stock
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Description

Contactin-1/CNTN1 proteins mediate cell surface interactions in the developing nervous system. Contactin-1/CNTN1 Protein, Human (HEK293, His) is the recombinant human-derived Contactin-1/CNTN1, expressed by HEK293 , with C-His labeled tag. The total length of Contactin-1/CNTN1 Protein, Human (HEK293, His) is 973 a.a.,

Background

Contactin-1 (CNTN1) protein, a key player in nervous system development, facilitates critical cell surface interactions. It plays a significant role in the formation of paranodal axo-glial junctions within myelinated peripheral nerves and contributes to signaling between axons and myelinating glial cells by associating with CNTNAP1. Additionally, CNTN1 participates in the generation of oligodendrocytes, acting as a ligand for NOTCH1. The association with NOTCH1 promotes the activation of NOTCH1, leading to the release of the notch intracellular domain (NICD) and its subsequent translocation to the nucleus. Notably, CNTN1's interaction with TNR induces neuron repulsion and inhibits neurite outgrowth. In its monomeric form, CNTN1 interacts with CNTNAP1 in cis, and it binds to the carbonic-anhydrase like domain of PTPRZ1. Furthermore, CNTN1 forms a complex with NRCAM and PTPRB and interacts with TASOR. These multifaceted interactions underscore the diverse functions of Contactin-1 in orchestrating crucial processes during nervous system development.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q12860 (E21-S993)

Gene ID
Molecular Construction
N-term
CNTN1 (E21-S993)
Accession # Q12860
His
C-term
Synonyms
CNTN1; Contactin1; Glycoprotein gp135; GP135
Molecular Weight

Approximately 125 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Contactin-1/CNTN1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Contactin-1/CNTN1 Protein, Human (HEK293, His)
Cat. No.:
HY-P74225
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