Contactin-2/CNTN2 Protein, Mouse (HEK293, His)

Contactin-2/CNTN2 Protein collaborates with transmembrane protein CNTNAP2, crucially organizing axonal domains at nodes of Ranvier.This partnership maintains voltage-gated potassium channels at the juxtaparanodal region, emphasizing Contactin-2's pivotal role in the intricate molecular architecture essential for efficient nerve impulse propagation along myelinated axons.Contactin-2/CNTN2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-2/CNTN2 protein, expressed by HEK293 , with C-His labeled tag.

Partnering with the transmembrane protein CNTNAP2, the Contactin-2/CNTN2 protein plays a crucial role in organizing axonal domains at nodes of Ranvier. Its collaborative action involves the maintenance of voltage-gated potassium channels specifically at the juxtaparanodal region. This dynamic interplay highlights Contactin-2's pivotal contribution to the intricate molecular architecture that underlies the proper functioning of nodes of Ranvier, essential for the efficient propagation of nerve impulses along myelinated axons.

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is ≤1.421 μg/mL, corresponding to a specific activity is ≥703.729 units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 1.421 μg/ml, corresponding to a specific activity is 703.729 units/mg.

Mouse

HEK293

C-10*His

Q61330 (Q31-E1013)

21367  [NCBI]

QGTPATFGPVFEEQPVGLLFPEESAEDQVTLACRARASPPATYRWKMNGTEMNLEPGSRHQLMGGNLVIMSPTKAQDAGVYQCLASNPVGTVVSKEAVLRFGFLQEFSKEERDPVKTHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCLATSHLDFSTKSVFSKFAQLNLAAEDPRLFAPSIKARFPPETYALVGQQVTLECFAFGNPVPRIKWRKVDGSLSPQWGTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGCAAAGKPRPMVRWLRNGEPLASQNRVEVLAGDLRFSKLNLEDSGMYQCVAENKHGTIYASAELAVQALAPDFRQNPVRRLIPAARGGEISIPCQPRAAPKATILWSKGTEILGNSTRVTVTLDGTLIIRNISRSDEGKYTCFAENFMGKANSTGILSVRDATKITLAPSSADINVGDNLTLQCHASHDPTMDLTFTWTLDDFPVDFDKPGGHYRRASVKETIGDLTILNAQLRHGGTYTCMAQTVVDGASKEATVLVRGPPGPPGGVVVRDIGDTTVQLSWSRGFDNHSPIAKYTLQARTPPSGKWKQVRTNPVNIEGNAETAQVLGLMPWMDYEFRVSASNILGTGEPSGPSSRIRTKEAVPSVAPSGLSGGGGAPGELTINWTPMSREYQNGDGFGYLLSFRRQGSSSWQTARVPGADTQYFVYSNDSIHPYTPFEVKIRSYNRRGDGPESLTAIVYSAEEEPKVAPAKVWAKGSSSSEMNVSWEPVLQDMNGILLGYEIRYWKAGDKEAAADRVRTAGLDSSARVTGLYPNTKYHVTVRAYNRAGTGPASPSADAMTMKPPPRRPPGNISWTFSSSSLSLKWDPVVPLRNESTVTGYKMLYQNDLQPTPMLHLTSKNWIEIPVPEDIGHALVQIRTTGPGGDGIPAEVHIVRNGGTSMMVE

Approximately 128-135 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.