1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Contactin-3
  6. Contactin-3/CNTN3 Protein, Mouse (HEK293, His)

Contactin-3/CNTN3 Protein, Mouse (HEK293, His)

Cat. No.: HY-P74228
COA Handling Instructions

Contactin-3/CNTN3 protein facilitates cell surface interactions in nervous system development, promoting neurite outgrowth and interacting with PTPRG to regulate neuronal growth and connectivity.Contactin-3/CNTN3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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10 μg $80 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Contactin-3/CNTN3 protein facilitates cell surface interactions in nervous system development, promoting neurite outgrowth and interacting with PTPRG to regulate neuronal growth and connectivity.Contactin-3/CNTN3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

Background

Contactin-3/CNTN3 protein plays a crucial role in facilitating cell surface interactions during nervous system development. It exhibits neurite outgrowth-promoting activity and interacts with PTPRG, indicating its involvement in signaling pathways that regulate neuronal growth and connectivity.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.097 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.097 μg/mL.
Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

Q07409 (E20-G1001)

Gene ID
Molecular Construction
N-term
CNTN3 (M1-G1001)
Accession # Q07409
His
C-term
Synonyms
Contactin-3; BIG-1; CNTN3; KIAA1496; PANG
AA Sequence

ELLLQGPVFIKEPSNSIFPVDSEDKKITLNCEARGNPSPHYRWQLNGSDIDTSLDHRYKLNGGNLIVINPNRNWDTGSYQCFATNSLGTIVSREAKLQFAYLENFKTRMRSTVSVREGQGVVLLCGPPPHSGELSYAWVFNEYPSFVEEDSRRFVSQETGHLYIAKVEPSDVGNYTCVVTSTVTNTRVLGSPTPLVLRSDGVMGEYEPKIEVQFPETLPAAKGSTVRLECFALGNPVPQINWRRSDGMPFPNKIKLRKFNGMLEIQNFQQEDTGSYECIAENSRGKNVARGRLTYYAKPYWLQLLRDVEIAVEDSLYWECRASGKPKPSYRWLKNGDALVLEERIQIENGALTITNLNVTDSGMFQCIAENKHGLIYSSAELKVVASAPDFSRNPMKKMVQVQVGSLVILDCKPRASPRALSFWKKGDMMVREQARVSFLNDGGLKIMNVTKADAGTYTCTAENQFGKANGTTHLVVTEPTRIILAPSNMDVAVGESVILPCQVQHDPLLDIMFAWYFNGALTDFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAAELIVRGSPGPPENVKVDEITDTTAQLSWTEGTDSHSPVISYAVQARTPFSVGWQSVRTVPEVIDGKTHTATVVELNPWVEYEFRIVASNKIGGGEPSLPSEKVRTEEAAPEIAPSEVSGGGGSRSELVITWDPVPEELQNGGGFGYVVAFRPLGVTTWIQTVVTSPDNPRYVFRNESIVPFSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSHISAHSLSSSEIEVSWNTIPWKLSNGHLLGYEVRYWNNGGEEESSRKVKVAGNQTSAVLRGLKSNLAYYTAVRAYNSAGAGPFSATVNATTKKTPPSQPPGNVVWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVHVLNTNKTSAELLLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARG

Molecular Weight

Approximately 130-160 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Contactin-3/CNTN3 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Contactin-3/CNTN3 Protein, Mouse (HEK293, His)
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