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  3. COX4NB Protein, Human (His)

COX4NB protein is a component of the endoplasmic reticulum membrane protein complex (EMC) and is able to insert newly synthesized membrane proteins into the endoplasmic reticulum membrane in an energy-independent manner. Its preference for transmembrane domains with weakly hydrophobic or unstable characteristics guides co- and post-translational insertion processes. COX4NB Protein, Human (His) is the recombinant human-derived COX4NB protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.

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Description

COX4NB protein is a component of the endoplasmic reticulum membrane protein complex (EMC) and is able to insert newly synthesized membrane proteins into the endoplasmic reticulum membrane in an energy-independent manner. Its preference for transmembrane domains with weakly hydrophobic or unstable characteristics guides co- and post-translational insertion processes. COX4NB Protein, Human (His) is the recombinant human-derived COX4NB protein, expressed by E. coli , with N-His labeled tag.

Background

COX4NB protein serves as a crucial component of the endoplasmic reticulum membrane protein complex (EMC), facilitating the energy-independent insertion of newly synthesized membrane proteins into endoplasmic reticulum (ER) membranes. It exhibits a preference for accommodating proteins with transmembrane domains characterized by weak hydrophobicity or containing destabilizing features such as charged and aromatic residues. COX4NB is actively involved in both cotranslational and post-translational insertion processes, playing a key role in the cotranslational insertion of multi-pass membrane proteins where stop-transfer membrane-anchor sequences transform into ER membrane-spanning helices. Additionally, it is essential for the post-translational insertion of tail-anchored (TA) proteins into ER membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, positioning the translocated N-terminus in the ER lumen, COX4NB controls the topology of multi-pass membrane proteins, including G protein-coupled receptors. Indirectly influencing various cellular processes through its regulatory role in protein membrane insertion, COX4NB is a vital component of the EMC complex, where it functions in conjunction with other subunits like EMC8 and EMC9.

Species

Human

Source

E. coli

Tag

N-His

Accession

O43402-1 (M1-C210)

Gene ID
Molecular Construction
N-term
His
COX4NB (M1-C210)
Accession # O43402-1
C-term
Synonyms
ER membrane protein complex subunit 8; Neighbor of COX4; C16orf2; COX4AL; COX4NB; FAM158B; NOC4
Molecular Weight

Approximately 27 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

COX4NB Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
COX4NB Protein, Human (His)
Cat. No.:
HY-P77342
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