CREG1/CREG Protein, Mouse (HEK293, His)

CREG1/CREG is a potential regulator in transcriptional control that antagonizes adenovirus E1A-induced activation, emphasizing its role in regulating cell growth and differentiation. The glycosylation-dependent interaction with IGF2R highlights the multifaceted nature of CREG1/CREG in the molecular mechanisms governing transcriptional control. CREG1/CREG Protein, Mouse (HEK293, His) is the recombinant mouse-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CREG1/CREG Protein, Mouse (HEK293, His) is 189 a.a., with molecular weight of 26-34 kDa.

The CREG1/CREG protein emerges as a potential regulator in the transcriptional control of cell growth and differentiation. Notably, it acts as an antagonist to transcriptional activation and cellular transformation induced by the adenovirus E1A protein. The transcriptional control activity associated with cell growth necessitates the interaction with IGF2R. CREG1/CREG forms homodimers, and its interaction with IGF2R is dependent on glycosylation. These characteristics underline the multifaceted nature of CREG1/CREG in molecular mechanisms involved in transcriptional control, emphasizing its potential significance in modulating cellular growth, differentiation, and responses to external stimuli. Further investigation is crucial to unravel the specific pathways and molecular events orchestrated by CREG1/CREG in these regulatory processes.

Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED₅₀ for this effect is 17.01 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED₅₀ for this effect is 17.01 ng/mL.

Mouse

HEK293

C-6*His

O88668 (R32-Q220)

433375  [NCBI]

RGGRDHGDWDVDRRLPPLPPREDGPRVARFVTHVSDWGSLATISTIKEVRGWPFADIISISDGPPGEGTGEPYMYLSPLQQAVSDLQENPEATLTMSLAQTVYCRNHGFDPQSPLCVHIMMSGTVTKVNKTEEDYARDSLFVRHPEMKHWPSSHNWFFAKLKISRIWVLDYFGGPKVVTPEEYFNVTLQ

26-34 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.