CRELD1 Protein, Human (HEK293, His)

CRELD1 Protein, acting as a protein disulfide isomerase, aids in the correct folding and assembly of proteins, crucial for cellular homeostasis. It also contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane, emphasizing its significance in maintaining protein structure and membrane receptor distribution. CRELD1 Protein, Human (HEK293, His) is the recombinant human-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.

CRELD1 protein, functioning as a protein disulfide isomerase, plays a role in cellular processes related to the correct folding and assembly of proteins. In addition, it contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane. The involvement of CRELD1 in these molecular mechanisms underscores its significance in cellular homeostasis, particularly in the context of protein structure and membrane receptor distribution.

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.

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  Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.

Human

HEK293

C-6*His

Q96HD1-1 (Q30-E362)

78987  [NCBI]

QPSPPPQSSPPPQPHPCHTCRGLVDSFNKGLERTIRDNFGGGNTAWEEENLSKYKDSETRLVEVLEGVCSKSDFECHRLLELSEELVESWWFHKQQEAPDLFQWLCSDSLKLCCPAGTFGPSCLPCPGGTERPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNASHLVCSACFGPCARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE

Approximately 45-50 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.