CRKL Protein, Human

CRKL protein mediates intracellular signal transduction, interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, and plays a role in erythropoietin receptor and phosphoinositide signaling. It also binds to DOCK2 and DOCK5, suggesting regulation of guanine nucleotide exchange factors. CRKL Protein, Human is the recombinant human-derived CRKL protein, expressed by E. coli , with tag free. The total length of CRKL Protein, Human is 303 a.a., .

The CRKL protein is implicated in mediating the transduction of intracellular signals, indicating its role in cellular signaling pathways. It interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, suggesting its involvement in pathways associated with erythropoietin receptor signaling and phosphoinositide signaling. Additionally, CRKL interacts with DOCK2 and DOCK5 via its first SH3 domain, highlighting its potential role in modulating guanine nucleotide exchange factors. The protein further engages with phosphorylated CBLB and IRS4, indicating its participation in pathways related to cellular proliferation and insulin receptor signaling. Furthermore, CRKL interacts with BCAR1/CAS and NEDD9/HEF1, suggesting its involvement in signaling cascades associated with cell adhesion and migration. These diverse interactions underscore the versatility of CRKL in transmitting signals within the cell.

Human

E. coli

Tag Free

P46109 (M1-E303)

1399

Approximately 33.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.