CSEN Protein, Human (His)

CSEN is a calcium-dependent transcriptional repressor that binds to the DRE elements of genes such as PDYN and FOS. Its affinity to DNA is affected by calcium. The binding force is reduced in the presence of calcium and enhanced when combined with magnesium. CSEN Protein, Human (His) is the recombinant human-derived CSEN protein, expressed by E. coli , with N-His labeled tag.

CSEN, a calcium-dependent transcriptional repressor, plays a multifaceted role in cellular processes. It binds to the DRE (downstream regulatory element) of various genes, including PDYN and FOS, exhibiting reduced affinity for DNA upon calcium binding, and enhanced affinity with magnesium. Additionally, CSEN is implicated in nociception, suggesting a potential involvement in pain perception. Beyond its transcriptional functions, CSEN acts as a regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. In this capacity, it modulates channel expression at the cell membrane, influencing gating characteristics, inactivation kinetics, and the rate of recovery from inactivation. Notably, these regulatory actions are exerted in a calcium-dependent and isoform-specific manner, underscoring the complexity of CSEN's role in potassium channel activity.

Human

E. coli

N-His

Q9Y2W7-1 (M1-I256)

30818  [NCBI]

MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI

Approximately 29 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.