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CSTL1 Protein, Human (HEK293, Fc)

Cat. No.: HY-P77344
COA Handling Instructions

Cystatin-like protein 1 (CSTL1) is a member of the cystatin family. CSTL1 Protein, Human (HEK293, Fc) is the recombinant human-derived CSTL1 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
Free Sample   Apply now
5 μg $40 In-stock
10 μg $70 In-stock
50 μg $200 In-stock
100 μg $340 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Cystatin-like protein 1 (CSTL1) is a member of the cystatin family. CSTL1 Protein, Human (HEK293, Fc) is the recombinant human-derived CSTL1 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CSTL1 Protein is a type 2 cystatin-like protein. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The specific function of this protein has not been determined. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q9H114 (A20-R145)

Gene ID

128817  [NCBI]

Molecular Construction
N-term
CSTL1 (A20-R145)
Accession # Q9H114
hFc
C-term
Synonyms
Cystatin-like 1; RCET11; CSTL1
AA Sequence

AKLGHFQRWEGFQQKLMSKKNMNSTLNFFIQSYNNASNDTYLYRVQRLIRSQMQLTTGVEYIVTVKIGWTKCKRNDTSNSSCPLQSKKLRKSLICESLIYTMPWINYFQLWNNSCLEAEHVGRNLR

Molecular Weight

Approximately 45-80 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CSTL1 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CSTL1 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P77344
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