CTLA-4 Protein, Human (HEK293, His)

The CTLA-4 protein is a key inhibitory receptor and a major negative regulator of T cell responses in coordination of immune regulation. Its unique property is its significantly increased affinity for B7 ligands (CD80/CD86) compared with CD28. CTLA-4 Protein, Human (HEK293, His) is the recombinant human-derived CTLA-4 protein, expressed by HEK293 , with C-6*His labeled tag.

GMP CTLA-4, a pivotal inhibitory receptor, emerges as a principal negative regulator orchestrating T-cell responses within the intricate framework of immune modulation. This regulatory function stems from the distinctive property of GMP CTLA-4, displaying significantly heightened affinity for its natural B7 family ligands, CD80 and CD86, compared to the cognate stimulatory coreceptor CD28. This pronounced difference in binding affinity positions GMP CTLA-4 to competitively engage with CD80/B7-1 and CD86/B7.2, exerting a suppressive influence on T-cell activation and finely tuning immune responses. The homodimeric structure of GMP CTLA-4, intricately linked by disulfide bonds, further underscores its role as a molecular sentinel in immune regulation. Additionally, GMP CTLA-4 interacts with ICOSLG, contributing to its multifaceted engagement in immune checkpoint pathways.

10 μg/mL (100 μL/well) of immobilized Mouse B7-1-Fc can bind Human CTLA-4-His with an ED₅₀ value of 5-50 ng/mL.

Human

HEK293

C-6*His

P16410 (K36-D161)

1493  [NCBI]

KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD

20-25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.