CYP51 Protein, Mycobacterium tuberculosis (sf9, His-Myc)

CYP51 Proteinas is a sterol 14α-demethylase that acts with an unknown physiological substrate and accepts electrons from the iron-sulfur ferredoxin Fdx. In vitro, it catalyzes the C14-demethylation of lanosterol, 24,25-dihydrolanosterol, and obtanyol, showing stereoselectivity in producing 8,14-diene. CYP51 Protein, Mycobacterium tuberculosis (sf9, His-Myc) is the recombinant CYP51 protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag.

CYP51 protein, identified as a sterol 14alpha-demethylase, operates with an as-yet-unknown physiological substrate and receives electrons from the iron-sulfur ferredoxin Fdx, encoded by an adjacent gene. In vitro experiments demonstrate its capability to catalyze the C14-demethylation of lanosterol, 24,25-dihydrolanosterol, and obtusifoliol, exhibiting stereoselectivity in producing the 8,14-dienes. This enzymatic activity underscores its pivotal role in sterol biosynthesis, particularly in generating essential intermediates such as 8,14-dienes.

Others

Sf9 insect cells

N-10*His;C-Myc

P9WPP8 (M1-V451)

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MSAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDECGDVGTFQLAGKQVVLLSGSHANEFFFRAGDDDLDQAKAYPFMTPIFGEGVVFDASPERRKEMLHNAALRGEQMKGHAATIEDQVRRMIADWGEAGEIDLLDFFAELTIYTSSACLIGKKFRDQLDGRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARNGLVALVADIMNGRIANPPTDKSDRDMLDVLIAVKAETGTPRFSADEITGMFISMMFAGHHTSSGTASWTLIELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLRLHPPLIILMRVAKGEFEVQGHRIHEGDLVAASPAISNRIPEDFPDPHDFVPARYEQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQIKAIFSVLLREYEFEMAQPPESYRNDHSKMVVQLAQPACVRYRRRTGV

Approximately 54.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.