Cystatin S/CST4 Protein, Human (HEK293, His)

Cystatin S (CST4) protein strongly inhibits papain and ficin, showing potent inhibition. Although it partially inhibits stem bromelain and bovine cathepsin C, it does not block the activity of porcine cathepsin B or clostridial protease. Cystatin S/CST4 Protein, Human (HEK293, His) is the recombinant human-derived Cystatin S/CST4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cystatin S/CST4 Protein, Human (HEK293, His) is 121 a.a., with molecular weight of ~16.0 kDa.

Cystatin S (CST4) protein exhibits robust inhibitory activity against papain and ficin enzymes, demonstrating a potent inhibitory effect. Additionally, it partially inhibits stem bromelain and bovine cathepsin C, though it does not impede the activities of porcine cathepsin B or clostripain. Notably, the inhibition of papain follows a non-competitive mode. This diverse inhibitory profile highlights the specificity of CST4 in regulating the activities of various proteases, suggesting its role in modulating proteolytic processes. The distinctive inhibitory properties of CST4 against specific enzymes contribute to its functional significance in cellular and physiological contexts, underscoring its potential impact on protease-mediated pathways and cellular homeostasis.

Human

HEK293

C-6*His

P01036 (S21-A141)

1472  [NCBI]

SSSKEENRIIPGGIYDADLNDEWVQRALHFAISEYNKATEDEYYRRPLQVLRAREQTFGGVNYFFDVEVGRTICTKSQPNLDTCAFHEQPELQKKQLCSFEIYEVPWEDRMSLVNSRCQEA

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 9.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.